Product Name: IL-4 Antibody [1.3_4F11-2G3]
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.0 ng/mL), a concentration of 6.0-8.5 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 0.5-1.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human IL-4 when used with our biotinylated antigen affinity purified anti-human IL-4 (XP-5188Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL.Western Blot:To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: The monoclonal antibody was produced in BALB/c x ICR F1 mice using recombinant hIL-4 as the immunizing antigen.
Host Species: Mouse
Purification: This IgG1k antibody was purified on a Protein A affinity column.
Physical State: Lyophilized
CAS NO.: 41621-49-2
Product: Amprolium (hydrochloride)
Buffer:
Concentration:
Storage Conditions: IL-4 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 – 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Interleukin 4Interleukin-4, B-cell stimulatory factor 1, IL-4, BSF1, BCGF1, BSF-1, BCGF-1
Accession NO.: P05112
Protein Ino: 124337
Official Symbol: IL4
Geneid: 3565
Background: IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
PubMed ID:http://aac.asm.org/content/26/4/507.abstract
