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Product Name :
anti-ubiquityl-histone h2b (lys120) mouse mab

Isotype :
IgG

Conjugate :
Unconjugated

Synonyms:
H2BK120ub

UniProt ID :
P62807

Immunogen:
Ubiquitinated human histone H2B (Lys120) peptide

MW (kDa) :
23

Specificity:
Anti-Ubiquityl-Histone H2B (Lys120) Mouse mAb detects histone H2B only when it is ubiquitinated at Lys120. This antibody has been shown to selectively recognize ubiquitinated H2B peptide at Lys120, but not the acetylated, succinylated, 2-hydroxyisobutyrylated, β-Hydroxybutyrylated, or unmodified H2B peptide at Lys120.

Purity :
Protein G purified

Purity :
PBS, Glycerol, BSA

Storage :
Store at -20°C. Avoid freeze/thaw cycles.

Stability:
Stable for 12 months from date of receipt/reconstitution.

Background :
Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a “trans-histone” modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity. Cellular location Nucleus

Images :
Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 15 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: HeLa, N2a, BRL, mouse liver, mouse kidney Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: 23 kDa Observed band size: 23 kDa

Vapor Pressure :
Anti-Ubiquityl-Histone H2B (Lys120) Mouse mAb Clone Number: MD :

Anti-Ubiquityl-Histone H2B (Lys120) Mouse mAb Clone Number: MD

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Betaine hydrochloride