Product Name :
anti-ubiquityl-histone h2b (lys120) mouse mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H2BK120ub
UniProt ID :
P62807
Immunogen:
Ubiquitinated human histone H2B (Lys120) peptide
MW (kDa) :
23
Specificity:
Anti-Ubiquityl-Histone H2B (Lys120) Mouse mAb detects histone H2B only when it is ubiquitinated at Lys120. This antibody has been shown to selectively recognize ubiquitinated H2B peptide at Lys120, but not the acetylated, succinylated, 2-hydroxyisobutyrylated, β-Hydroxybutyrylated, or unmodified H2B peptide at Lys120.
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a “trans-histone” modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity. Cellular location Nucleus
Images :
Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 15 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: HeLa, N2a, BRL, mouse liver, mouse kidney Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: 23 kDa Observed band size: 23 kDa
Vapor Pressure :
Anti-Ubiquityl-Histone H2B (Lys120) Mouse mAb Clone Number: MD
Anti-Ubiquityl-Histone H2B (Lys120) Mouse mAb Clone Number: MD
Related websites: https://www.medchemexpress.com/antibodies.html
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