anti-phospho-akt (thr308) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phospho-AKT (Thr308) Rabbit mAb Clone Number: JMMR-3567 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Mouse Synonyms: PKB beta, AKT2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms PKB beta, AKT2 UniProt ID P31751 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Phosphorylation on Thr-308, Ser-473 and Tyr-474 of AKT are required for its full activity. AKT1 Thr-308 and Ser-473 is Phosphorylated by IKBKE and TBK1. Cellular location Nucleus, Cell membrane, Cytoplasm, Endosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: NIH/3T3, 2: NIH/3T3 + PDGF(100ng/mL, 15 min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 56 kDa Observed band size: 60 kDa :
Recombinant Rabbit Monoclonal Anti-Phospho-AKT (Thr308) Rabbit mAb Clone Number: JMMR-3567 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Mouse Synonyms: PKB beta, AKT2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms PKB beta, AKT2 UniProt ID P31751 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Phosphorylation on Thr-308, Ser-473 and Tyr-474 of AKT are required for its full activity. AKT1 Thr-308 and Ser-473 is Phosphorylated by IKBKE and TBK1. Cellular location Nucleus, Cell membrane, Cytoplasm, Endosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: NIH/3T3, 2: NIH/3T3 + PDGF(100ng/mL, 15 min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 56 kDa Observed band size: 60 kDa