Product Name :
anti-pgp9.5 mouse mab
Isotype :
IgG2a/kappa
Conjugate :
Unconjugated
Synonyms:
UCHL1, UCH-L1, PGP 9.5
UniProt ID :
P09936
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Cellular location Cytoplasm, Endoplasmic reticulum, Membrane
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: SH-SY5Y, U87MG, Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 27 kDaObserved band size: 27 kDa ICC/IF Cell line: SH-SY5YFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Anti-PGP9.5 Mouse mAb Clone Number: 12635 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: UCHL1, UCH-L1, PGP 9.5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms UCHL1, UCH-L1, PGP 9.5 UniProt ID P09936 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Cellular location Cytoplasm, Endoplasmic reticulum, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: SH-SY5Y, U87MG, Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 27 kDaObserved band size: 27 kDa ICC/IF Cell line: SH-SY5YFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Recombinant Anti-PGP9.5 Mouse mAb Clone Number: 12635 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: UCHL1, UCH-L1, PGP 9.5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms UCHL1, UCH-L1, PGP 9.5 UniProt ID P09936 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Cellular location Cytoplasm, Endoplasmic reticulum, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: SH-SY5Y, U87MG, Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 27 kDaObserved band size: 27 kDa ICC/IF Cell line: SH-SY5YFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
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