anti-crotonyllysine mouse mab

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Anti-Crotonyllysine Mouse mAb Clone Number: 4D5 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Kcr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kcr UniProt ID / Immunogen Crotonylated lysine peptides MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lysine crotonylation is a newly-identified histone post_x0002_translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 100 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: HeLa Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: Multiple kDa Observed band size: Multiple kDa :
Anti-Crotonyllysine Mouse mAb Clone Number: 4D5 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Kcr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kcr UniProt ID / Immunogen Crotonylated lysine peptides MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lysine crotonylation is a newly-identified histone post_x0002_translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 100 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: HeLa Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: Multiple kDa Observed band size: Multiple kDa