anti-histone h3 rabbit mab (hrp conjugate)

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Recombinant Rabbit Monoclonal Anti-Histone H3 Rabbit mAb (HRP Conjugate) Clone Number: 8-H2L4 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate HRP Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d UniProt ID P68431 Immunogen Synthetic peptide corresponding to the N-terminus of human histone H3 protein MW (kDa) 15 Specificity Anti-Histone H3 Rabbit mAb (HRP Conjugate) detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:30000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:30000 Primary Ab incubation condition: 2 hours at room temperature Lysate: 1: HeLa, 2: HEK-293, 3: NIH/3T3, 4: PC-12, 5: Mouse brain, 6: Rat brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human kidney Section type: Formalin fixed & Paraffin – embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Histone H3 Rabbit mAb (HRP Conjugate) Clone Number: 8-H2L4 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate HRP Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d UniProt ID P68431 Immunogen Synthetic peptide corresponding to the N-terminus of human histone H3 protein MW (kDa) 15 Specificity Anti-Histone H3 Rabbit mAb (HRP Conjugate) detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:30000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:30000 Primary Ab incubation condition: 2 hours at room temperature Lysate: 1: HeLa, 2: HEK-293, 3: NIH/3T3, 4: PC-12, 5: Mouse brain, 6: Rat brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human kidney Section type: Formalin fixed & Paraffin – embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with