anti-phospho-axl (tyr702) rabbit pab

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Anti-Phospho-Axl (Tyr702) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: UFO, AXL oncogene Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms UFO, AXL oncogene UniProt ID P30530 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Axl, also known as Ufo or Ark, is a member of receptor tyrosine kinase of the TAM family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl.Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): H1299, (+): H1299+pervanadate (3mM, 30min)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 98 kDaObserved band size: 130 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): PC-3, (+): PC-3 + pervanadate (10 μM, 30min)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 98 kDaObserved band size: 130 kDa :
Anti-Phospho-Axl (Tyr702) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: UFO, AXL oncogene Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms UFO, AXL oncogene UniProt ID P30530 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Axl, also known as Ufo or Ark, is a member of receptor tyrosine kinase of the TAM family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl.Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): H1299, (+): H1299+pervanadate (3mM, 30min)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 98 kDaObserved band size: 130 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): PC-3, (+): PC-3 + pervanadate (10 μM, 30min)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 98 kDaObserved band size: 130 kDa