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Zeng et al. AMB Expr (2017) 7:154 DOI ten.1186/s13568-017-0451-ORIGINAL ARTICLEOpen AccessMutagenesis and characterization of a Bacillus amyloliquefaciens strain for Cinnamomum camphora seed kernel oil extraction by aqueous enzymatic methodCheng Zeng1,two,three, Rongbin Zhao1,3,4, Maomao Ma1,3,five, Zheling Zeng1,three,four and Deming Gong1,three,6*Abstract The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N-nitro-N-nitroso guanidine treatment, to be able to improve the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic system (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31.9 compared using the parental strain. The extracellular enzymes developed by NCU116-1 included proteinase, pectase, glucoamylase, cellulase and amylase. The proteinase had the maximum activity at 50 . Its optimum temperature and pH worth were around 45 and 8.0 respectively. Mn2+ was an activator of neutral proteinase. The glucoamylase had the maximum activity at 35 , and was activated by Cu2+, Fe3+ and Mn2+. Its optimum temperatures and pH worth were 35 and eight.0 respectively. The pectinase had the maximum activity at 40 , and was activated by Ca2+ and Mn2+. Its optimum temperatures and pH worth were 350 and six.0 respectively. The optimum conditions of CCSKO extraction by AEM were also investigated. The results suggested that the most beneficial volume of enzyme solution and enzymolysis time had been 20 (v/v) and 4 h, respectively. The oil extraction rate was 95.2 beneath these situations. As a result, a suitable mutated strain was chosen for CCSKO extraction by AEM and also the optimum extraction conditions had been determined. Search phrases: Bacillus amyloliquefacien, UV and NTG mutation, Proteinase, Enzymatic properties, Aqueous enzymatic strategy Introduction Cinnamomum camphora (Lauraceae) is usually a plant with high economic values (Babu et al. 2003). The content material of medium chain fatty acid triglyceride (MCT) in Cinnamomum camphora seed kernel oil (CCSKO) was higher (MCT 94 ), and MCT was reported to cut down the consumption and subsequent weight achieve of animals (Mumme and Stonehouse 2015; Ferreira et al. 2013; Wang et al.GAS6 Protein medchemexpress 2016) and increase blood lipid and*Correspondence: dgong01@gmail.MIP-1 alpha/CCL3 Protein MedChemExpress com Cheng Zeng and Rongbin Zhao contributed equally to this work 6 New Zealand Institute of All-natural Medicine Investigation, eight Ha Crescent, Auckland 2104, New Zealand Full list of author information and facts is accessible in the end of your articleglucose levels (Page et al.PMID:24140575 2009; Fu et al. 2015). There has been a expanding interest in CCSKO and its extraction. Meanwhile, MCT was identified to possess a powerful inhibitory impact on Gram-positive bacteria, Gram-negative bacteria, mould and enzymes (Hui et al. 2009; Luo et al. 2014). In industrial processes, edible oil was obtained by pressing, or a process that combines pressing and organic solvent (Rosenthal et al. 2001). In consideration of environmental protection, aqueous enzymatic process (AEM) has been well accepted by researchers. The power consumption of edible oil extraction by AEM was low and the method was protected, which was recogn.
