Product Name :
anti-phosphotyrosine rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
pTyr
UniProt ID :
/
Immunogen:
MW (kDa) :
Multiple
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Tyrosine phosphorylation is one of the major means of cell signaling transduction in multiple pathophysiological conditions regulated by tyrosine kinase activities. In cancer, unregulated tyrosine kinase activity can drive tumor malignancy and formation by generating inappropriate proliferation and survival signals. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to these studies. The anti-phosphotyrosine antibody (clone 3B6) developed by PTM Biolabs, Inc. provides an exceptionally sensitive tool for studying tyrosine phosphorylation and its kinase activities in biomedical and preclinic studies as well as high throughput drug discovery. Cellular location /
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Sodium pervanadate (3mM, 30min)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: Multiple kDaObserved band size: Multiple kDa IHC-P Tissue: Mouse kidneySection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Phosphotyrosine Rabbit mAb Clone Number: PF
Recombinant Rabbit Monoclonal Anti-Phosphotyrosine Rabbit mAb Clone Number: PF
Related websites: https://www.medchemexpress.com/antibodies.html
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