anti-qsox1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-QSOX1 Rabbit mAb Clone Number: JRMR-10186-22 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB FC IP Reactivity: Human Synonyms: QSOX1, QSCN6 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms QSOX1, QSCN6 UniProt ID O00391 Immunogen Recombinant human QSOX1 fragment MW (kDa) 67 Specificity Anti-QSOX1 Rabbit mAb recognizes endogeneous level of QSOX1 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human FC 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background QSOX1 belongs to the family of FAD-dependent sulfhydryl oxidases and is an atypical disulfide catalyst. QSOX1 is mainly located in the Golgi apparatus and endoplasmic reticulum (ER) in human embryonic fibroblasts, and it cooperates with protein disulfide isomerase (PDI) to help fold new-born proteins in the cell. QSOX1 is mainly expressed in the heart, placenta, lung, liver, skeletal muscle, and pancreas, and is very weakly expressed in the brain and kidneys. Studies have found that the increase of QSOX1 expression in tumor cells enables tumor cells to actively circumvent the mechanism of apoptosis mediated by reactive oxygen species. Cellular location Extracellular area, Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: MDA-MB-231, 3: T47D, 4: 293TProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 83 kDaObserved band size: 67 kDa FC Cell line: MDA-MB-231Fixative: 4% ParaformaldehydePermeabilization: 90% MethanolPrimary Ab dilution: 1:100Secondary Ab: Goat anti Rabbit IgGUnlabeled control: Cells without incubation with primary antibody and secondary antibody (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for :
Recombinant Rabbit Monoclonal Anti-QSOX1 Rabbit mAb Clone Number: JRMR-10186-22 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB FC IP Reactivity: Human Synonyms: QSOX1, QSCN6 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms QSOX1, QSCN6 UniProt ID O00391 Immunogen Recombinant human QSOX1 fragment MW (kDa) 67 Specificity Anti-QSOX1 Rabbit mAb recognizes endogeneous level of QSOX1 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human FC 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background QSOX1 belongs to the family of FAD-dependent sulfhydryl oxidases and is an atypical disulfide catalyst. QSOX1 is mainly located in the Golgi apparatus and endoplasmic reticulum (ER) in human embryonic fibroblasts, and it cooperates with protein disulfide isomerase (PDI) to help fold new-born proteins in the cell. QSOX1 is mainly expressed in the heart, placenta, lung, liver, skeletal muscle, and pancreas, and is very weakly expressed in the brain and kidneys. Studies have found that the increase of QSOX1 expression in tumor cells enables tumor cells to actively circumvent the mechanism of apoptosis mediated by reactive oxygen species. Cellular location Extracellular area, Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: MDA-MB-231, 3: T47D, 4: 293TProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 83 kDaObserved band size: 67 kDa FC Cell line: MDA-MB-231Fixative: 4% ParaformaldehydePermeabilization: 90% MethanolPrimary Ab dilution: 1:100Secondary Ab: Goat anti Rabbit IgGUnlabeled control: Cells without incubation with primary antibody and secondary antibody (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for