anti-alpha tubulin mouse mab

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Recombinant Anti-alpha Tubulin Mouse mAb Clone Number: 11340 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: TUBA4A, TUBA1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TUBA4A, TUBA1 UniProt ID P68366 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human ICC/IF 1:100 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Cellular location Cytoplasm, Cytoskeleton, Microtubule Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: HeLa, 2: DaudiProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 50 kDaObserved band size: 55 kDa ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Anti-alpha Tubulin Mouse mAb Clone Number: 11340 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: TUBA4A, TUBA1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TUBA4A, TUBA1 UniProt ID P68366 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human ICC/IF 1:100 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Cellular location Cytoplasm, Cytoskeleton, Microtubule Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: HeLa, 2: DaudiProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 50 kDaObserved band size: 55 kDa ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with