anti-histone h3 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Histone H3 Rabbit mAb Clone Number: JMMR-2423B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d UniProt ID P68431 Immunogen Synthetic peptide derived from human histone H3 protein MW (kDa) 15 Specificity Anti-Histone H3 Rabbit mAb detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat, Monkey IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: NIH/3T3, 3: PC-12, 4: COS-7 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Histone H3 Rabbit mAb Clone Number: JMMR-2423B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d UniProt ID P68431 Immunogen Synthetic peptide derived from human histone H3 protein MW (kDa) 15 Specificity Anti-Histone H3 Rabbit mAb detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat, Monkey IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: NIH/3T3, 3: PC-12, 4: COS-7 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with