anti-ogt mouse mab

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Recombinant Anti-OGT Mouse mAb Clone Number: 11768 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms / UniProt ID O15294 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the ‘Thr-308’ phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Cellular location Cytoplasm, Membrane, Mitochondrion, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, 293, PC-12Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 110 kDaObserved band size: 110 kDa IHC-P Tissue: Human lung squamous carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-OGT Mouse mAb Clone Number: 11768 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms / UniProt ID O15294 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the ‘Thr-308’ phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Cellular location Cytoplasm, Membrane, Mitochondrion, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, 293, PC-12Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 110 kDaObserved band size: 110 kDa IHC-P Tissue: Human lung squamous carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with