Product Name :
anti-propionyl-histone h3 (lys56) rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K56pr
UniProt ID :
P68431
Immunogen:
MW (kDa) :
15
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H4 at Lys5, propionylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:5000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ng, 64ngExposure time: 30 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+sodium butyrate (30mM, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidneyProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa IHC-P Tissue: Human prostateSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary ab dilution: 1:500Primary ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Propionyl-Histone H3 (Lys56) Rabbit mAb Clone Number:
Recombinant Rabbit Monoclonal Anti-Propionyl-Histone H3 (Lys56) Rabbit mAb Clone Number:
Related websites: https://www.medchemexpress.com/antibodies.html
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