anti-cbp rabbit pab

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Anti-CBP Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: CREBBP, CREB-binding protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CREBBP, CREB-binding protein UniProt ID Q92793 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery. CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins. Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors. Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ. Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex. CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS. The role of acetylation of CBP/p300 is of particular interest. Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: HeLa Protein loading amount: 20 μg Exposure time: 30 secondsPredicted band size: 265 kDa Observed band size: 240 kDa :
Anti-CBP Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: CREBBP, CREB-binding protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CREBBP, CREB-binding protein UniProt ID Q92793 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery. CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins. Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors. Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ. Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex. CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS. The role of acetylation of CBP/p300 is of particular interest. Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: HeLa Protein loading amount: 20 μg Exposure time: 30 secondsPredicted band size: 265 kDa Observed band size: 240 kDa