Product Name :
anti-occludin mouse mab
Isotype :
IgG1/kappa
Conjugate :
Unconjugated
Synonyms:
OCLN
UniProt ID :
Q16625
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Occludin is a component of tight junctions. Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1. VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability. Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV). Cellular location Cell membrane
Images :
WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: 293, 2: HUVEC, 3: Caco-2 Protein loading quantity: 20 μg Exposure time: 1, 2: 10 s, 3: 60 secondsPredicted band size: 59 kDa Observed band size: 59 kDa ICC/IF Cell line: Caco-2Fixative: 100% Ice-cold ethanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Anti-Occludin Mouse mAb Clone Number: 13187 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: OCLN Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms OCLN UniProt ID Q16625 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Occludin is a component of tight junctions. Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1. VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability. Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV). Cellular location Cell membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: 293, 2: HUVEC, 3: Caco-2 Protein loading quantity: 20 μg Exposure time: 1, 2: 10 s, 3: 60 secondsPredicted band size: 59 kDa Observed band size: 59 kDa ICC/IF Cell line: Caco-2Fixative: 100% Ice-cold ethanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Recombinant Anti-Occludin Mouse mAb Clone Number: 13187 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: OCLN Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms OCLN UniProt ID Q16625 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Occludin is a component of tight junctions. Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1. VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability. Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV). Cellular location Cell membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: 293, 2: HUVEC, 3: Caco-2 Protein loading quantity: 20 μg Exposure time: 1, 2: 10 s, 3: 60 secondsPredicted band size: 59 kDa Observed band size: 59 kDa ICC/IF Cell line: Caco-2Fixative: 100% Ice-cold ethanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Alkaline Phosphatase Antibody
CDK5 Antibody (YA796)
SREBP1 Antibody: SREBP1 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 122 kDa, targeting to SREBP1. It can be used for WB,ELISA assays with tag free, in the background of Human, Mouse, Rat.
