Product Name :
anti-phospho-histone h3 (thr11) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3T11ph
UniProt ID :
P68431
Immunogen:
MW (kDa) :
15
Specificity:
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H3 phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbi IgG H&L (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 17 kDaObserved band size: 17 kDa
Vapor Pressure :
Anti-Phospho-Histone H3 (Thr11) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications: WB Reactivity: Human, Mouse, Rat Synonyms: H3T11ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3T11ph UniProt ID P68431 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H3 phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbi IgG H&L (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 17 kDaObserved band size: 17 kDa
Anti-Phospho-Histone H3 (Thr11) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications: WB Reactivity: Human, Mouse, Rat Synonyms: H3T11ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3T11ph UniProt ID P68431 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H3 phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbi IgG H&L (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 17 kDaObserved band size: 17 kDa
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