Product Name :
anti-pin1 rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
/
UniProt ID :
Q9QUR7
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Pin1 is a member of the parvulin family of peptidyl-prolyl isomerases (PPIase). It has been implicated in the G2/M transition of the mammalian cell cycle. Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules. Cellular location Nucleus, Cytoplasm
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse skin (Low expression), 2: Mouse brainProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 18 kDaObserved band size: 18 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse spleen, 2: NIH/3T3, 3: PC_x005f_x0002_12, 4: MCF-7, 5: HeLa Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 18 kDaObserved band size: 18 kDa ICC/IF Cell line: HelaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Pin1 Rabbit mAb Clone Number: JRMR-11025-13 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID Q9QUR7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Pin1 is a member of the parvulin family of peptidyl-prolyl isomerases (PPIase). It has been implicated in the G2/M transition of the mammalian cell cycle. Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse skin (Low expression), 2: Mouse brainProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 18 kDaObserved band size: 18 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse spleen, 2: NIH/3T3, 3: PC_x005f_x0002_12, 4: MCF-7, 5: HeLa Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 18 kDaObserved band size: 18 kDa ICC/IF Cell line: HelaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with
Recombinant Rabbit Monoclonal Anti-Pin1 Rabbit mAb Clone Number: JRMR-11025-13 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID Q9QUR7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Pin1 is a member of the parvulin family of peptidyl-prolyl isomerases (PPIase). It has been implicated in the G2/M transition of the mammalian cell cycle. Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse skin (Low expression), 2: Mouse brainProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 18 kDaObserved band size: 18 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse spleen, 2: NIH/3T3, 3: PC_x005f_x0002_12, 4: MCF-7, 5: HeLa Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 18 kDaObserved band size: 18 kDa ICC/IF Cell line: HelaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with
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