anti-histone h3.3 (mutated k36m) rabbit pab

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Anti-Histone H3.3 (Mutated K36M) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: H3-3A, H3.3A, H3F3, H3F3A, H3-3B, H3.3B, H3F3B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3-3A, H3.3A, H3F3, H3F3A, H3-3B, H3.3B, H3F3B UniProt ID P84243 Immunogen Synthetic peptide corresponding to human histone H3.3 K36M mutant MW (kDa) 15 Specificity Anti-Histone H3.3 (Mutated K36M) Rabbit pAb detects endogenous levels of K36M mutants of histone H3.1, H3.2, and H3.3 proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background K36M mutant of histone H3.3 is an “oncohistone” identified in more than 90% of chondroblastoma, a rare type of benign tumor caused by cartilage-producing cells. The mutant inhibits at least two H3K36 methyltransferases MMSET/WHSC1 and SETD2, triggering a depletion of H3K36 methylation and an increase of H3K27 trimethylation. H3.3K36M mutant proteins further alters expression of various genes including BMP2 that regulate oncogenesis and chondrogenesis. Chondrocytes expressing H3.3K36M exhibit several cancer-associated cellular phenotypes, including increased ability to form colonies, resistance to staurosporine-induced apoptosis, and defects in differentiation. Cellular location Nucleus Images WB Lysate: 1: Whole cell lysate of HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag. 2: Whole cell lysate of HEK-293T transfected with Histone H3.3 K36M (mutatnt) expression vector containing a myc-His-tag. Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 15 kDa :
Anti-Histone H3.3 (Mutated K36M) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: H3-3A, H3.3A, H3F3, H3F3A, H3-3B, H3.3B, H3F3B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3-3A, H3.3A, H3F3, H3F3A, H3-3B, H3.3B, H3F3B UniProt ID P84243 Immunogen Synthetic peptide corresponding to human histone H3.3 K36M mutant MW (kDa) 15 Specificity Anti-Histone H3.3 (Mutated K36M) Rabbit pAb detects endogenous levels of K36M mutants of histone H3.1, H3.2, and H3.3 proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background K36M mutant of histone H3.3 is an “oncohistone” identified in more than 90% of chondroblastoma, a rare type of benign tumor caused by cartilage-producing cells. The mutant inhibits at least two H3K36 methyltransferases MMSET/WHSC1 and SETD2, triggering a depletion of H3K36 methylation and an increase of H3K27 trimethylation. H3.3K36M mutant proteins further alters expression of various genes including BMP2 that regulate oncogenesis and chondrogenesis. Chondrocytes expressing H3.3K36M exhibit several cancer-associated cellular phenotypes, including increased ability to form colonies, resistance to staurosporine-induced apoptosis, and defects in differentiation. Cellular location Nucleus Images WB Lysate: 1: Whole cell lysate of HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag. 2: Whole cell lysate of HEK-293T transfected with Histone H3.3 K36M (mutatnt) expression vector containing a myc-His-tag. Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 15 kDa