anti-phospho-vimentin (ser39) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phospho-Vimentin (Ser39) Rabbit mAb Clone Number: JMMR-3651 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: VIM Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms VIM UniProt ID P08670 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA) and inhibited by the antiinflammatory cytokine IL10, suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Cellular location Cytoplasm, Cytoskeleton, Nucleus, Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: (-) HeLa, 2: (+) HeLa + calyculinA(100nM, 30min), 3: (-) NIH/3T3, 4: (+) NIH/3T3+calyculin A (100nM, 30min), 5: (-) C6, 6: (+) C6+ calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 54 kDa Observed band size: 56 kDa :
Recombinant Rabbit Monoclonal Anti-Phospho-Vimentin (Ser39) Rabbit mAb Clone Number: JMMR-3651 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: VIM Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms VIM UniProt ID P08670 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA) and inhibited by the antiinflammatory cytokine IL10, suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Cellular location Cytoplasm, Cytoskeleton, Nucleus, Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: (-) HeLa, 2: (+) HeLa + calyculinA(100nM, 30min), 3: (-) NIH/3T3, 4: (+) NIH/3T3+calyculin A (100nM, 30min), 5: (-) C6, 6: (+) C6+ calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 54 kDa Observed band size: 56 kDa