anti-rab5a mouse mab

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Recombinant Anti-Rab5A Mouse mAb Clone Number: JMMR-3590 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Ras-related protein Rab-5A, RAB5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Ras-related protein Rab-5A, RAB5 UniProt ID P20339 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background RAB5A is a member of the Ras superfamily of small Rab GTPases. RAB5A is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis. The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis. RAB5A exerts its function by interacting with several Rab5-specific effectors. These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of RAB5A-cargo targets between endosome and the plasma membrane. Cellular location Cell membrane, Cytoplasm, Endosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: K562, 2: NIH/3T3, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 24 kDa Observed band size: 24 kDa IHC-P Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-Rab5A Mouse mAb Clone Number: JMMR-3590 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Ras-related protein Rab-5A, RAB5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Ras-related protein Rab-5A, RAB5 UniProt ID P20339 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background RAB5A is a member of the Ras superfamily of small Rab GTPases. RAB5A is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis. The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis. RAB5A exerts its function by interacting with several Rab5-specific effectors. These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of RAB5A-cargo targets between endosome and the plasma membrane. Cellular location Cell membrane, Cytoplasm, Endosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: K562, 2: NIH/3T3, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 24 kDa Observed band size: 24 kDa IHC-P Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with