anti-2-hydroxyisobutyryllysine mouse mab

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Anti-2-Hydroxyisobutyryllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Khib Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Khib UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 4 ng, 20 ng, 100 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: HeLa, N2a, BRL, Mouse liver Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: Multiple kDa Observed band size: Multiple kDa :
Anti-2-Hydroxyisobutyryllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Khib Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Khib UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 4 ng, 20 ng, 100 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: HeLa, N2a, BRL, Mouse liver Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: Multiple kDa Observed band size: Multiple kDa