anti-ccr6 rabbit mab

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Recombinant Rabbit Monoclonal Anti-CCR6 Rabbit mAb Clone Number: JRMR-10338-34 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: DRY6, CD196, CKRL3, CMKBR6, GPR29, STRL22 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms DRY6, CD196, CKRL3, CMKBR6, GPR29, STRL22 UniProt ID P51684 Immunogen Recombinant human CCR6 fragment MW (kDa) 42 Specificity Anti-CCR6 Rabbit mAb recognizes endogeneous level of CCR6 protein. Product Usage Information Applications Dilution Recommended Species WB 1:25000 – 1:50000 Human IHC-P 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity. Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: Raji, 3: HL-60 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 42 kDa Observed band size: 42 kDa IHC-P Tissue: Human tonsilSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-CCR6 Rabbit mAb Clone Number: JRMR-10338-34 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: DRY6, CD196, CKRL3, CMKBR6, GPR29, STRL22 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms DRY6, CD196, CKRL3, CMKBR6, GPR29, STRL22 UniProt ID P51684 Immunogen Recombinant human CCR6 fragment MW (kDa) 42 Specificity Anti-CCR6 Rabbit mAb recognizes endogeneous level of CCR6 protein. Product Usage Information Applications Dilution Recommended Species WB 1:25000 – 1:50000 Human IHC-P 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity. Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: Raji, 3: HL-60 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 42 kDa Observed band size: 42 kDa IHC-P Tissue: Human tonsilSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for