anti-sam68 rabbit pab

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Anti-SAM68 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: KHDRBS1, p68 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms KHDRBS1, p68 UniProt ID Q07666 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. KHDRBS1 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing. KHDRBS1 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation. KHDRBS1 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of KHDRBS1 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA. Many of the activities of KHDRBS1 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where KHDRBS1 is frequently upregulated. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: HCT-116Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 68 kDaObserved band size: 68 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-SAM68 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: KHDRBS1, p68 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms KHDRBS1, p68 UniProt ID Q07666 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. KHDRBS1 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing. KHDRBS1 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation. KHDRBS1 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of KHDRBS1 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA. Many of the activities of KHDRBS1 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where KHDRBS1 is frequently upregulated. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: HCT-116Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 68 kDaObserved band size: 68 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with