Product Name :
anti-phospho-histone h1.b (thr146) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H1.BT146ph
UniProt ID :
P10412
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+ Nocodazole(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 33 kDaObserved MW: 33 kDa IP IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extractsIP ab incubation condition:
Vapor Pressure :
Anti-Phospho-Histone H1.B (Thr146) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB、IP Reactivity: Human, Mouse, Rat Synonyms: H1.BT146ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H1.BT146ph UniProt ID P10412 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human，Mouse，Rat IP 1:25-1:100 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+ Nocodazole(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 33 kDaObserved MW: 33 kDa IP IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extractsIP ab incubation condition:
Anti-Phospho-Histone H1.B (Thr146) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB、IP Reactivity: Human, Mouse, Rat Synonyms: H1.BT146ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H1.BT146ph UniProt ID P10412 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human，Mouse，Rat IP 1:25-1:100 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+ Nocodazole(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 33 kDaObserved MW: 33 kDa IP IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extractsIP ab incubation condition:
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Caspase-14 Antibody
Histone H2B Antibody
LAMP2a Antibody: LAMP2a Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 45 kDa, targeting to LAMP2a. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human, Mouse, Rat.
