anti-rock2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-ROCK2 Rabbit mAb Clone Number: 12619 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: ROCK-II, p164 ROCK-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms ROCK-II, p164 ROCK-2 UniProt ID O75116 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human IP 1:25 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Rho associated coiled-coil containing protein kinase 2 is a protein that in humans is encoded by the ROCK2 gene. Fasudil is an inhibitor of ROCK protein.The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. Cellular location Membrane, Cytoplasm, Cytoskeleton, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLaProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 160 kDaObserved band size: 190-200 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: A549, 2: Mouse brain, 3: Rat brain, 4: SH-SY5YProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 160 kDaObserved band size: 190-200 kDa IHC-P Tissue: Human colon cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-ROCK2 Rabbit mAb Clone Number: 12619 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: ROCK-II, p164 ROCK-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms ROCK-II, p164 ROCK-2 UniProt ID O75116 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human IP 1:25 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Rho associated coiled-coil containing protein kinase 2 is a protein that in humans is encoded by the ROCK2 gene. Fasudil is an inhibitor of ROCK protein.The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. Cellular location Membrane, Cytoplasm, Cytoskeleton, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLaProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 160 kDaObserved band size: 190-200 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: A549, 2: Mouse brain, 3: Rat brain, 4: SH-SY5YProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 160 kDaObserved band size: 190-200 kDa IHC-P Tissue: Human colon cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with