anti-phospho-histone h3 (ser28) mouse mab

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Anti-Phospho-Histone H3 (Ser28) Mouse mAb Clone Number: 12385 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3S28ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/Kappa Conjugate Unconjugated Synonyms H3S28ph UniProt ID P68431 Immunogen Phosphorylated human histone H3 (Ser28) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+Nocodazole(100ng/ml, 18hr)Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 15 kDaObserved band size: 15 kDa ChIP Cell type: HeLa+ Nocodazole (100 ng/ml, 18 hr)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×10*6 cellsAmount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, LDHA and LDHA-promoter. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-Phospho-Histone H3 (Ser28) Mouse mAb Clone Number: 12385 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3S28ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/Kappa Conjugate Unconjugated Synonyms H3S28ph UniProt ID P68431 Immunogen Phosphorylated human histone H3 (Ser28) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+Nocodazole(100ng/ml, 18hr)Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 15 kDaObserved band size: 15 kDa ChIP Cell type: HeLa+ Nocodazole (100 ng/ml, 18 hr)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×10*6 cellsAmount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, LDHA and LDHA-promoter. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.