anti-nse rabbit pab

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Anti-NSE Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC Reactivity: Human, Mouse, Rat Synonyms: ENO2, Enolase 2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms ENO2, Enolase 2 UniProt ID P09104 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat FC 1:50 – 1:100 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Neuron specific enolase (NSE), also known as enolase 2 (ENO2) or gamma enolase, is an enzyme that is encoded by the ENO2 gene in human. NSE is a phosphopyruvate hydratase. NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: HeLa, 2: U87-MG, 3: RajiProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 47 kDaObserved band size: 47 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: Mouse liver, 2: Mouse brain, 3: Rat brainProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 47 kDaObserved band size: 47 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: SH-SY5YProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 47 kDaObserved band size: 47 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 90% Methanol permeabilizationPrimary Ab dilution: 1:100Secondary Ab: Goat Anti-Rabbit IgGUnlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for :
Anti-NSE Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC Reactivity: Human, Mouse, Rat Synonyms: ENO2, Enolase 2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms ENO2, Enolase 2 UniProt ID P09104 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat FC 1:50 – 1:100 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Neuron specific enolase (NSE), also known as enolase 2 (ENO2) or gamma enolase, is an enzyme that is encoded by the ENO2 gene in human. NSE is a phosphopyruvate hydratase. NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: HeLa, 2: U87-MG, 3: RajiProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 47 kDaObserved band size: 47 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: Mouse liver, 2: Mouse brain, 3: Rat brainProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 47 kDaObserved band size: 47 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: SH-SY5YProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 47 kDaObserved band size: 47 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 90% Methanol permeabilizationPrimary Ab dilution: 1:100Secondary Ab: Goat Anti-Rabbit IgGUnlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for