anti-no66 mouse mab

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Recombinant Anti-NO66 Mouse mAb Clone Number: 13000 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: RIOX1, C14orf169, MAPJD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms RIOX1, C14orf169, MAPJD UniProt ID Q9H6W3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates ‘Lys-4’ (H3K4me) and ‘Lys-36’ (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 ‘Lys-4’ (H3K4me3) and monomethylated H3 ‘Lys-4’ (H3K4me1) residues, while it has weaker activity for dimethylated H3 ‘Lys-36’ (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on ‘His-216’. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Lysate: HeLa Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 75 kDa Observed band size: 80 kDa IHC-P Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Description: The brown color represents the positive signal observed with :
Recombinant Anti-NO66 Mouse mAb Clone Number: 13000 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: RIOX1, C14orf169, MAPJD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms RIOX1, C14orf169, MAPJD UniProt ID Q9H6W3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates ‘Lys-4’ (H3K4me) and ‘Lys-36’ (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 ‘Lys-4’ (H3K4me3) and monomethylated H3 ‘Lys-4’ (H3K4me1) residues, while it has weaker activity for dimethylated H3 ‘Lys-36’ (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on ‘His-216’. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Lysate: HeLa Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 75 kDa Observed band size: 80 kDa IHC-P Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Description: The brown color represents the positive signal observed with