anti-pax9 rabbit mab

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Recombinant Rabbit Monoclonal Anti-PAX9 Rabbit mAb Clone Number: 11453 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms / UniProt ID P55771 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Paired box gene 9, also known as PAX9, is a protein which in humans is encoded by the PAX9 gene. This gene is a member of the paired box (PAX) family of transcription factors. During mouse embryogenesis Pax9 expression starts from embryonic day 8.5 and becomes more evident by E9.5; at this stage its expression is restricted to the pharyngeal endoderm. Later on, Pax9 is also expressed in the axial skeleton. Pax9 is required for craniofacial, tooth and limb development, and may more generally involve development of stratified squamous epithelia as well as various organs and skeletal elements. PAX9 plays a role in the absence of wisdom teeth in some human populations. It is a transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: PC-3, 2: Jurkat, 3: Rat thymus Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 36 kDa IHC-P Tissue: Human breast carcinoma Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-PAX9 Rabbit mAb Clone Number: 11453 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms / UniProt ID P55771 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Paired box gene 9, also known as PAX9, is a protein which in humans is encoded by the PAX9 gene. This gene is a member of the paired box (PAX) family of transcription factors. During mouse embryogenesis Pax9 expression starts from embryonic day 8.5 and becomes more evident by E9.5; at this stage its expression is restricted to the pharyngeal endoderm. Later on, Pax9 is also expressed in the axial skeleton. Pax9 is required for craniofacial, tooth and limb development, and may more generally involve development of stratified squamous epithelia as well as various organs and skeletal elements. PAX9 plays a role in the absence of wisdom teeth in some human populations. It is a transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: PC-3, 2: Jurkat, 3: Rat thymus Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 36 kDa IHC-P Tissue: Human breast carcinoma Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with