anti-s100b rabbit pab

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Anti-S100B Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: S-100 protein beta chain, S100 calcium-binding protein B, S100 beta Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms S-100 protein beta chain, S100 calcium-binding protein B, S100 beta UniProt ID P04271 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:1000 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background S100B weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. S100B binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. The interaction between S100B and AGER after MI may play a role in apoptosis by activating ERK1/2 and p53 / TP53 signaling. S100B could assist ATAD3A cytoplasmic processing,preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: T47D (Negative control), 2: SK-MEL-28Protein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 10 kDa Observed band size: 10 kDa IHC-P Tissue: Human skin squamous cell carcinoma Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Anti-S100B Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: S-100 protein beta chain, S100 calcium-binding protein B, S100 beta Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms S-100 protein beta chain, S100 calcium-binding protein B, S100 beta UniProt ID P04271 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:1000 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background S100B weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. S100B binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. The interaction between S100B and AGER after MI may play a role in apoptosis by activating ERK1/2 and p53 / TP53 signaling. S100B could assist ATAD3A cytoplasmic processing,preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: T47D (Negative control), 2: SK-MEL-28Protein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 10 kDa Observed band size: 10 kDa IHC-P Tissue: Human skin squamous cell carcinoma Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with