anti-perilipin-2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Perilipin-2 Rabbit mAb Clone Number: JMMR-3520 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: PLIN2, ADFP Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms PLIN2, ADFP UniProt ID Q99541 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background ADFP also known as ADRP (adipocyte differentiation related protein) , adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. Cellular location Lipid droplet Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 48 kDa Observed band size: 48 kDa ICC/IF Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Perilipin-2 Rabbit mAb Clone Number: JMMR-3520 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: PLIN2, ADFP Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms PLIN2, ADFP UniProt ID Q99541 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background ADFP also known as ADRP (adipocyte differentiation related protein) , adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. Cellular location Lipid droplet Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 48 kDa Observed band size: 48 kDa ICC/IF Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with