anti-s100a10 mouse mab

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Recombinant Anti-S100A10 Mouse mAb Clone Number: JMMR-1272D Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: Protein S100-A10, Calpactin I light chain, S100 calcium-binding protein A10, p10 protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Protein S100-A10, Calpactin I light chain, S100 calcium-binding protein A10, p10 protein UniProt ID P60903 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background S100A10 (also known as p11 or calpactin 1 light chain) forms a constitutive heterotetramer with annexin-A2 (ANXA2) and may act as a bridge between the plasma membrane and actin cytoskeleton via interactions with the plasma membrane (via ANXA2) and various protein partners such as the SNARE complex or actin. S100A10 has been hypothesized to play a critical role in neuronal signaling due to its interaction and regulation of neurotransmitter receptors and neuron-specific ion channels such as 5-HT1B, TRPV5, ASIC1, TASK1 and NaV1.8 . More recently, S100A10 has also been shown to modulate macrophage activation and invasion via its ability to bind and transmit receptor-like signals in response to plasminogen. Cellular location Plasma Membrane, Nucleus, secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: HT-29, 2: A431Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin-fixed & Paraffin -embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-S100A10 Mouse mAb Clone Number: JMMR-1272D Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: Protein S100-A10, Calpactin I light chain, S100 calcium-binding protein A10, p10 protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Protein S100-A10, Calpactin I light chain, S100 calcium-binding protein A10, p10 protein UniProt ID P60903 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background S100A10 (also known as p11 or calpactin 1 light chain) forms a constitutive heterotetramer with annexin-A2 (ANXA2) and may act as a bridge between the plasma membrane and actin cytoskeleton via interactions with the plasma membrane (via ANXA2) and various protein partners such as the SNARE complex or actin. S100A10 has been hypothesized to play a critical role in neuronal signaling due to its interaction and regulation of neurotransmitter receptors and neuron-specific ion channels such as 5-HT1B, TRPV5, ASIC1, TASK1 and NaV1.8 . More recently, S100A10 has also been shown to modulate macrophage activation and invasion via its ability to bind and transmit receptor-like signals in response to plasminogen. Cellular location Plasma Membrane, Nucleus, secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: HT-29, 2: A431Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin-fixed & Paraffin -embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with