Product Name :
anti-propionyl-histone h4 (lys5) rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H4K5pr
UniProt ID :
P62805
Immunogen:
Propionylated human histone H4 (Lys5) peptide
MW (kDa) :
11
Specificity:
Anti-Propionyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is propionylated at Lys5
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H4 at Lys5, propionylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 16 ng, 64ngExposure time: 10 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, HeLa+ sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, NIH-3T3, BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human prostate hyperplasiaSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Propionyl-Histone H4 (Lys5) Rabbit mAb Clone Number: 14H4L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: H4K5pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5pr UniProt ID P62805 Immunogen Propionylated human histone H4 (Lys5) peptide MW (kDa) 11 Specificity Anti-Propionyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is propionylated at Lys5 Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H4 at Lys5, propionylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 16 ng, 64ngExposure time: 10 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, HeLa+ sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, NIH-3T3, BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human prostate hyperplasiaSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Recombinant Rabbit Monoclonal Anti-Propionyl-Histone H4 (Lys5) Rabbit mAb Clone Number: 14H4L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: H4K5pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5pr UniProt ID P62805 Immunogen Propionylated human histone H4 (Lys5) peptide MW (kDa) 11 Specificity Anti-Propionyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is propionylated at Lys5 Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H4 at Lys5, propionylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 16 ng, 64ngExposure time: 10 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, HeLa+ sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, NIH-3T3, BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human prostate hyperplasiaSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
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