anti-pkc delta rabbit mab

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Recombinant Rabbit Monoclonal Anti-PKC delta Rabbit mAb Clone Number: 11553 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: nPKC-delta, SDK1, PKCD, PRKCD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms nPKC-delta, SDK1, PKCD, PRKCD UniProt ID Q05655 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PKC-delta(Protein kinase C delta type) is an enzyme that is encoded by the PRKCD gene in human. PKC is a family of serine- and threonine-specific protein kinases that can be activated by the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC-delta is one of the PKC family members. Studies both in human and mice demonstrate that PKC-delta is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PKC-delta is also regulated by phosphorylation on various serine/threonine and tyrosine residues including Y311. Cellular location Cytoplasm, Membrane, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: Rat brainProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 78 kDaObserved band size: 80 kDa ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanolPermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-PKC delta Rabbit mAb Clone Number: 11553 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: nPKC-delta, SDK1, PKCD, PRKCD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms nPKC-delta, SDK1, PKCD, PRKCD UniProt ID Q05655 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PKC-delta(Protein kinase C delta type) is an enzyme that is encoded by the PRKCD gene in human. PKC is a family of serine- and threonine-specific protein kinases that can be activated by the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC-delta is one of the PKC family members. Studies both in human and mice demonstrate that PKC-delta is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PKC-delta is also regulated by phosphorylation on various serine/threonine and tyrosine residues including Y311. Cellular location Cytoplasm, Membrane, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: Rat brainProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 78 kDaObserved band size: 80 kDa ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanolPermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with