anti-ppp1ca mouse mab

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Recombinant Anti-PPP1CA Mouse mAb Clone Number: JMMR-2727 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: PP-1A, PPP1A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms PP-1A, PPP1A UniProt ID P62136 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PPP1CA(protein phosphatase 1-alpha catalytic subunit) is a serine / threonine – protein phosphatase which is encoded by the PPP1CA gene in human. The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility and protein synthesis. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293T, 3: NIH/3T3, 4: Rat brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 38 kDa Observed band size: 38 kDa ICC/IF Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-PPP1CA Mouse mAb Clone Number: JMMR-2727 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: PP-1A, PPP1A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms PP-1A, PPP1A UniProt ID P62136 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PPP1CA(protein phosphatase 1-alpha catalytic subunit) is a serine / threonine – protein phosphatase which is encoded by the PPP1CA gene in human. The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility and protein synthesis. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293T, 3: NIH/3T3, 4: Rat brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 38 kDa Observed band size: 38 kDa ICC/IF Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with