anti-ppt1 mouse mab

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Recombinant Anti-PPT1 Mouse mAb Clone Number: 10732 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: CLN1, PPT, Palmitoyl-protein thioesterase 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms CLN1, PPT, Palmitoyl-protein thioesterase 1 UniProt ID P50897 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with the molecular mass of 32 kDa, 34 kDa,36 kDa and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form. Cellular location Lysosome, Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HepG2; 2: SK-BR-3 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 34 kDa Observed band size: 32 kDa :
Recombinant Anti-PPT1 Mouse mAb Clone Number: 10732 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: CLN1, PPT, Palmitoyl-protein thioesterase 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms CLN1, PPT, Palmitoyl-protein thioesterase 1 UniProt ID P50897 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with the molecular mass of 32 kDa, 34 kDa,36 kDa and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form. Cellular location Lysosome, Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HepG2; 2: SK-BR-3 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 34 kDa Observed band size: 32 kDa