anti-alpha smooth muscle actin rabbit mab

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Recombinant Rabbit Monoclonal Anti-alpha Smooth Muscle Actin Rabbit mAb Clone Number: JRMR-10178(2)-13 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human, Mouse, Rat Synonyms: Alpha-actin-2, ACTA2, ACTSA, ACTVS, α-SMA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Alpha-actin-2, ACTA2, ACTSA, ACTVS, α-SMA UniProt ID P62736 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:1000 – 1:5000 Human, Mouse, Rat ICC/IF 1:50 Human, Rat FC 1:50 – 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause a variety of myopathies, including nemaline myopathy, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects with manifestations such as hypotonia. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: HeLa, 3: A431, 4: HEK_x005f_x0002_293, 5: NIH-3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 42 kDaObserved band size: 42 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat colon, 2: Mouse colonProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 42 kDaObserved band size: 42 kDa IHC-P Tissue: Human prostatic hyperplasiaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:5000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-alpha Smooth Muscle Actin Rabbit mAb Clone Number: JRMR-10178(2)-13 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human, Mouse, Rat Synonyms: Alpha-actin-2, ACTA2, ACTSA, ACTVS, α-SMA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Alpha-actin-2, ACTA2, ACTSA, ACTVS, α-SMA UniProt ID P62736 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:1000 – 1:5000 Human, Mouse, Rat ICC/IF 1:50 Human, Rat FC 1:50 – 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause a variety of myopathies, including nemaline myopathy, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects with manifestations such as hypotonia. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: HeLa, 3: A431, 4: HEK_x005f_x0002_293, 5: NIH-3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 42 kDaObserved band size: 42 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat colon, 2: Mouse colonProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 42 kDaObserved band size: 42 kDa IHC-P Tissue: Human prostatic hyperplasiaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:5000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with