anti-phospho-beta catenin (ser37) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phospho-beta Catenin (Ser37) Rabbit mAb Clone Number: JRMR-3662 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: CTNNB1, CTNNB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CTNNB1, CTNNB UniProt ID P35222 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background β-catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. Cellular location Membrane, Cytoplasm, Cytoskeleton, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: (-) 293T; 2: (+) 293T+calyculin A (100nM, 30min)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 85 kDaObserved band size: 105 kDa :
Recombinant Rabbit Monoclonal Anti-Phospho-beta Catenin (Ser37) Rabbit mAb Clone Number: JRMR-3662 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: CTNNB1, CTNNB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CTNNB1, CTNNB UniProt ID P35222 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background β-catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. Cellular location Membrane, Cytoplasm, Cytoskeleton, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: (-) 293T; 2: (+) 293T+calyculin A (100nM, 30min)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 85 kDaObserved band size: 105 kDa