anti-phospho-caveolin-1 (tyr14) rabbit pab

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Anti-Phospho-Caveolin-1 (Tyr14) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: Human Synonyms: CAV1, CAV Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CAV1, CAV UniProt ID Q03135 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:10000-1:50000 Human ICC/IF 1:10000-1:50000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor. Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma. However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer. Recent study also show that CAV1 is involved in astric Cancer. MW of Caveolin-1 is from 20-25 kDa due to phosphorylation. Cellular location Cell membrane, Golgi apparatus, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50,000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): A431, (+): A431 +pervanadate (1mM, 30min)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 20 kDaObserved band size: 23, 25 kDa ICC/IF Cell line: (A) A431, (B) A431 + Pervanadate (1mM,30min)Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with :
Anti-Phospho-Caveolin-1 (Tyr14) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: Human Synonyms: CAV1, CAV Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CAV1, CAV UniProt ID Q03135 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:10000-1:50000 Human ICC/IF 1:10000-1:50000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor. Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma. However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer. Recent study also show that CAV1 is involved in astric Cancer. MW of Caveolin-1 is from 20-25 kDa due to phosphorylation. Cellular location Cell membrane, Golgi apparatus, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50,000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): A431, (+): A431 +pervanadate (1mM, 30min)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 20 kDaObserved band size: 23, 25 kDa ICC/IF Cell line: (A) A431, (B) A431 + Pervanadate (1mM,30min)Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with