anti-propionyl-histone h2a (lys125) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Propionyl-Histone H2A (Lys125) Rabbit mAb Clone Number: PA-076-08 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: Human Synonyms: H2AK125pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK125pr UniProt ID P04908 Immunogen Propionylated human histone H2A (Lys125) peptide MW (kDa) 14 Specificity Anti-Propionyl-Histone H2A (Lys125) Rabbit mAb detects histone H2A only when it is propionylated at Lys125, but not the crotonylated and unmodified H2A peptide at Lys125. Product Usage Information Applications Dilution Recommended Species WB 1:2000 – 1:10000 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H2A at Lys125 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 30 s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-):MCF-7, (+):MCF-7+Sodium Butyrate(50mM, 24hr)+Trichostatin A(500ng-ml, 4hr)Protein loading amount: 20 μgExposure time: 30 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+Sodium Butyrate(30mM, 4hr)Protein loading amount: 20 μgExposure time: 30 sPredicted MW: 14 kDaObserved MW: 14 kDa ChIP Cell type: HeLa treated with Sodium butyrate (5mM, 24hr)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A MagBeadsDescription: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA CDS region, FOXO3a-promoter, FOXO3a-downstream, RAB20 and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Recombinant Rabbit Monoclonal Anti-Propionyl-Histone H2A (Lys125) Rabbit mAb Clone Number: PA-076-08 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: Human Synonyms: H2AK125pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK125pr UniProt ID P04908 Immunogen Propionylated human histone H2A (Lys125) peptide MW (kDa) 14 Specificity Anti-Propionyl-Histone H2A (Lys125) Rabbit mAb detects histone H2A only when it is propionylated at Lys125, but not the crotonylated and unmodified H2A peptide at Lys125. Product Usage Information Applications Dilution Recommended Species WB 1:2000 – 1:10000 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H2A at Lys125 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 30 s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-):MCF-7, (+):MCF-7+Sodium Butyrate(50mM, 24hr)+Trichostatin A(500ng-ml, 4hr)Protein loading amount: 20 μgExposure time: 30 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+Sodium Butyrate(30mM, 4hr)Protein loading amount: 20 μgExposure time: 30 sPredicted MW: 14 kDaObserved MW: 14 kDa ChIP Cell type: HeLa treated with Sodium butyrate (5mM, 24hr)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A MagBeadsDescription: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA CDS region, FOXO3a-promoter, FOXO3a-downstream, RAB20 and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.