anti-cbp rabbit mab

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Recombinant Rabbit Monoclonal Anti-CBP Rabbit mAb Clone Number: 10707 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: CREBBP, CREB-binding protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CREBBP, CREB-binding protein UniProt ID Q92793 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene.The CREB protein carries out its function by activating transcription, where interaction with transcription factors is managed by one or more CREB domains: the nuclear receptor interaction domain (RID), the KIX domain (CREB and MYB interaction domain), the cysteine/histidine regions (TAZ1/CH1 and TAZ2/CH3) and the interferon response binding domain. The CREB protein domains, KIX, TAZ1 and TAZ2, each bind tightly to a sequence spanning both transactivation domains 9aaTADs of transcription factor p53. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: 293T nuclear, 2: 293T cytosol, 3: SH-SY5Y, 4: C6 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 300 kDa Observed band size: 300 kDa ICC/IF Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CBP Rabbit mAb Clone Number: 10707 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: CREBBP, CREB-binding protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CREBBP, CREB-binding protein UniProt ID Q92793 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene.The CREB protein carries out its function by activating transcription, where interaction with transcription factors is managed by one or more CREB domains: the nuclear receptor interaction domain (RID), the KIX domain (CREB and MYB interaction domain), the cysteine/histidine regions (TAZ1/CH1 and TAZ2/CH3) and the interferon response binding domain. The CREB protein domains, KIX, TAZ1 and TAZ2, each bind tightly to a sequence spanning both transactivation domains 9aaTADs of transcription factor p53. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: 293T nuclear, 2: 293T cytosol, 3: SH-SY5Y, 4: C6 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 300 kDa Observed band size: 300 kDa ICC/IF Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with