anti-pkm2 mouse mab

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Recombinant Anti-PKM2 Mouse mAb Clone Number: 11614 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CTHBP, OIP-3, Pyruvate kinase 2/3, THBP1, Tumor M2-PK, p58 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms CTHBP, OIP-3, Pyruvate kinase 2/3, THBP1, Tumor M2-PK, p58 UniProt ID P14618 Immunogen Recombinant human PKM2 fragment MW (kDa) 58 Specificity Anti-PKM2 Mouse mAb recognizes endogenous level of PKM2 protein. Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PKM2, also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone – binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene. PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: MCF7, 3: C6, 4: Mouse brain Protein loading amount: 20 μg Exposure time: 1-3: 10 s, 4: 30 secondsPredicted band size: 58 kDa Observed band size: 60 kDa ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-PKM2 Mouse mAb Clone Number: 11614 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CTHBP, OIP-3, Pyruvate kinase 2/3, THBP1, Tumor M2-PK, p58 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms CTHBP, OIP-3, Pyruvate kinase 2/3, THBP1, Tumor M2-PK, p58 UniProt ID P14618 Immunogen Recombinant human PKM2 fragment MW (kDa) 58 Specificity Anti-PKM2 Mouse mAb recognizes endogenous level of PKM2 protein. Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PKM2, also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone – binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene. PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: MCF7, 3: C6, 4: Mouse brain Protein loading amount: 20 μg Exposure time: 1-3: 10 s, 4: 30 secondsPredicted band size: 58 kDa Observed band size: 60 kDa ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with