anti-redd1 mouse mab

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Recombinant Anti-REDD1 Mouse mAb Clone Number: 13402 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: REDD-1, DDIT4, RTP801 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms REDD-1, DDIT4, RTP801 UniProt ID Q9NX09 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background REDD1 is a protein that in humans is encoded by the DDIT4 gene. It regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. And REDD1 plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Its role in the response to hypoxia depends on the cell type. Cellular location Cytoplasm, Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: K-562, 2: HepG2+CoCl2(-), 3: HepG2+CoCl2(+) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 25 kDa Observed band size: 30-35 kDa ICC/IF Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-REDD1 Mouse mAb Clone Number: 13402 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: REDD-1, DDIT4, RTP801 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms REDD-1, DDIT4, RTP801 UniProt ID Q9NX09 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background REDD1 is a protein that in humans is encoded by the DDIT4 gene. It regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. And REDD1 plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Its role in the response to hypoxia depends on the cell type. Cellular location Cytoplasm, Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: K-562, 2: HepG2+CoCl2(-), 3: HepG2+CoCl2(+) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 25 kDa Observed band size: 30-35 kDa ICC/IF Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with