anti-phospho-cdcp1 (tyr743) rabbit pab

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Anti-Phospho-CDCP1 (Tyr743) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: SIMA135, CD318, TRASK Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SIMA135, CD318, TRASK UniProt ID Q9H5V8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 0.7361111111111112 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CUB domain containing protein 1, also named as CDCP1 or SIMA135, is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons. Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression. The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions. Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins. Cellular location Cell membrane, Membrane, Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): PC-3, (+): PC-3 + Sodium pervanadate (10μM, 30min)Protein loading amount: 20 μgExposure time: 30 sPredicted band size: 93 kDaObserved band size: 140,70 kDa :
Anti-Phospho-CDCP1 (Tyr743) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: SIMA135, CD318, TRASK Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SIMA135, CD318, TRASK UniProt ID Q9H5V8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 0.7361111111111112 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CUB domain containing protein 1, also named as CDCP1 or SIMA135, is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons. Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression. The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions. Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins. Cellular location Cell membrane, Membrane, Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): PC-3, (+): PC-3 + Sodium pervanadate (10μM, 30min)Protein loading amount: 20 μgExposure time: 30 sPredicted band size: 93 kDaObserved band size: 140,70 kDa