Rochloride, an antiarrythmia drug in interlayer gallery of Na-clay (montmorillonite). The
Rochloride, an antiarrythmia drug in interlayer gallery of Na-clay (montmorillonite). The microcomposite particles ready from Abl Storage & Stability procainamide-montmorillonite hybrid and poly L-lactide were characterised by scanning electron microscope and atomic force microscopy analysis. In vitro drug release study in simulated intestinal fluid showed controlled release pattern as much as 72 h and important reduction in the drug release in gastric atmosphere. In vivo pharmacokinetics and biodistribution in rats showed that the plasmatissue drug levels were within therapeutic window as compared with cost-free drug. The information from toxicity biomarker estimations and clinical biochemistry haematological parameters showed important reduction in drug toxicity when formulated in montmorillonitepoly L-lactide as compared with cost-free drug, which is of considerable value in achieving improved therapy with lowered unwanted side effects. Crucial words: Antiarrythmia, controlled release, microcomposites, procainamide, toxicity biomarkerLayered silicates are emerging as promising candidates for applications in biomedical study encompassing drug delivery[1-5], tissue engineering[6,7], protein adsorption [8-11] , gene reservoirs and delivery[12,13] and nanoclay composites resulting from their ultra fine sizes are beneficial in tissue engineering applications [14-16], biocompatibility and controlled release of drug[4,5,14-16]. For EGFR/ErbB1/HER1 custom synthesis delivery applications, the layered silicates are best model for high amount of controlled release of drug and biomolecules, strength and null toxicity[4-5,14-18]. The purpose of this study was to use montmorillonite Na-clay (MMT) as carrier for controlled releases of procainamide hydrochloride (PA) and to achieve a delivery profile that would yield a higher blood level of the drug more than a extended time period and nullify toxicity of drug. Herein we report intercalation of PA in clay interlayer gallery of MMT to overcome drug toxicity and to preserve peak plasma drugAddress for correspondence E-mail: hcbajajcsmcri.orgconcentration by controlled release, measured by way of in vivo biomarker assessments. For the present study, procainamide HCl, poly L-lactide (PLLA) (inherent viscosity 0.90-1.20) and cellulose acetate dialysis tube (cut-off Mw: 7014) have been procured from Sigma-Aldrich, St Louis USA. Dichloromethane (DCM) and polyvinyl alcohol (Mw: 125 000) had been bought from S. D. FineChem. Ltd., India. Pentobarbital sodium was purchased from National Chemicals, Vadodara, India. The MMT-rich bentonite was collected from Akli mines, Barmer district, Rajasthan, India and purified. PA-MMT sample in bulk was prepared as was reported earlier[4]. All of the other reagents have been of HPLC grade and have been applied as received. The microcomposite particles (MPs) have been prepared using the oil in water (ow) solvent evaporation strategy. One gram of PLLA was dissolved in 100 ml DCM and sonicated for 20 min at 35(VWR Ultrasonic Cleaner, VWR International, Pennsylvania, USA), right after which PA-MMT hybrid (PLLA:PANovember – DecemberIndian Journal of Pharmaceutical SciencesijpsonlineMMT=1:0.five ww) was suspended within this organic phase and additional sonicated for ten min at 35 The organic phase was added drop wise (0.5 mlmin) into the external aqueous phase containing 0.5 wv of polyvinyl alcohol (300 ml) with stirring till DCM evaporation. The MPs had been collected and lyophilised in liquid nitrogen.In vitro release of PA was carried out with all the assistance of USP eight stage dissolution price test apparatus (Veego, India) us.
