anti-prc1 mouse mab

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Recombinant Anti-PRC1 Mouse mAb Clone Number: 10834 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: Protein regulator of cytokinesis 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Protein regulator of cytokinesis 1 UniProt ID O43663 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein Regulator of Cytokinesis 1 (PRC1) is a member of the MAP65/ASE1 family of nonmotor microtubule-associated proteins, first described in budding yeast. PRC1 is a substrate of CDK1, which maintains PRC1 in an inactive, monomeric state. Cell-cycle dependent degradation of CDK1 leads to dephosphorylation of PRC1 and subsequent KIF4-mediated translocation to the plus ends of microtubules, where it promotes microtubule bundling by cross-linking antiparallel microtubules. The microtubule bundling functions of PRC1 play a critical role in maintaining structural integritry of the spindle midzone during cytokinesis. Cellular location Nucleus, Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa; 2: 293T Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 72 kDa Observed band size: 66-72 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:200 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-PRC1 Mouse mAb Clone Number: 10834 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: Protein regulator of cytokinesis 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Protein regulator of cytokinesis 1 UniProt ID O43663 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein Regulator of Cytokinesis 1 (PRC1) is a member of the MAP65/ASE1 family of nonmotor microtubule-associated proteins, first described in budding yeast. PRC1 is a substrate of CDK1, which maintains PRC1 in an inactive, monomeric state. Cell-cycle dependent degradation of CDK1 leads to dephosphorylation of PRC1 and subsequent KIF4-mediated translocation to the plus ends of microtubules, where it promotes microtubule bundling by cross-linking antiparallel microtubules. The microtubule bundling functions of PRC1 play a critical role in maintaining structural integritry of the spindle midzone during cytokinesis. Cellular location Nucleus, Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa; 2: 293T Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 72 kDa Observed band size: 66-72 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:200 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with