Product Name :
anti-ubiquityl-histone h2a (lys119) rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H2AK119ub
UniProt ID :
P04908
Immunogen:
Ubiquitinated human histone H2A (Lys119) peptide
MW (kDa) :
23
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage. Cellular location Nucleus
Images :
WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: C2C12 Protein loading amount: 20 μg Exposure time: 3 s Predicted MW: 23 kDa Observed MW: 23 kDa ICC/IF Cell line: HeLa Fixation: 4% PFA Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:100 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Comment: P IP P of HeLa cells extracts: IP Ab incubation condition:
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb Clone Number: JMMR-N1208 Host: Rabbit Clonality: Recombinant Monoclonal Applications: WB ICC/IF IP ChIP Reactivity: Human, Mouse Synonyms: H2AK119ub Product Size 100μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK119ub UniProt ID P04908 Immunogen Ubiquitinated human histone H2A (Lys119) peptide MW (kDa) 23 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse ICC/IF 1:100 – 1:200 Human IP 1:50 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: C2C12 Protein loading amount: 20 μg Exposure time: 3 s Predicted MW: 23 kDa Observed MW: 23 kDa ICC/IF Cell line: HeLa Fixation: 4% PFA Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:100 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Comment: P IP P of HeLa cells extracts: IP Ab incubation condition:
Recombinant Rabbit Monoclonal Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb Clone Number: JMMR-N1208 Host: Rabbit Clonality: Recombinant Monoclonal Applications: WB ICC/IF IP ChIP Reactivity: Human, Mouse Synonyms: H2AK119ub Product Size 100μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK119ub UniProt ID P04908 Immunogen Ubiquitinated human histone H2A (Lys119) peptide MW (kDa) 23 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse ICC/IF 1:100 – 1:200 Human IP 1:50 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: C2C12 Protein loading amount: 20 μg Exposure time: 3 s Predicted MW: 23 kDa Observed MW: 23 kDa ICC/IF Cell line: HeLa Fixation: 4% PFA Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:100 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Comment: P IP P of HeLa cells extracts: IP Ab incubation condition:
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