anti-phospho-histone h2a (ser129) rabbit pab

Vapor Pressure :
Anti-Phospho-Histone H2A (Ser129) Rabbit pAb Clone Number: PA-157-B1195 Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: S.cerevisiae Synonyms: scH2AS129ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms scH2AS129ph UniProt ID P04911 Immunogen MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 S. cerevisiae Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the aminoterminal of core histones. This histone mark plays roles in DNA repair,transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. H2A(X) Ser 129 is essential for survival in the presence of topoisomerase-1-mediated DNA damage. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2: 64 ng Exposure time: 10 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: 1: Saccharomyces cerevisiae lysate, then the membrane treated with lambdaprotein phosphatase (400 U/ml) at 30­°C for 1 hour; 2: Saccharomyces cerevisiae lysate Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 14kDa Observed band size: 14kDa :
Anti-Phospho-Histone H2A (Ser129) Rabbit pAb Clone Number: PA-157-B1195 Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: S.cerevisiae Synonyms: scH2AS129ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms scH2AS129ph UniProt ID P04911 Immunogen MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 S. cerevisiae Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the aminoterminal of core histones. This histone mark plays roles in DNA repair,transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. H2A(X) Ser 129 is essential for survival in the presence of topoisomerase-1-mediated DNA damage. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2: 64 ng Exposure time: 10 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: 1: Saccharomyces cerevisiae lysate, then the membrane treated with lambdaprotein phosphatase (400 U/ml) at 30­°C for 1 hour; 2: Saccharomyces cerevisiae lysate Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 14kDa Observed band size: 14kDa