anti-qki rabbit mab

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Recombinant Rabbit Monoclonal Anti-QKI Rabbit mAb Clone Number: JMMR-3191 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Protein quaking, Hqk, HqkI Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Protein quaking, Hqk, HqkI UniProt ID Q96PU8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:3000 Human, Mouse, Rat ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background QKI is a member of the signal transduction and activator of RNA (STAR) protein family of RNA-binding proteins. Mutations in the mouse Qki locus results in impaired myelin formation resulting in tremors. QKI proteins exist as homodimers, and disruption of the dimerization process is lethal in mice. QKI exists in different isoforms that differ in their C-terminus, resulting in unique subcellular localizations. The nuclear isoform of QKI, QKI-5, is involved in regulation of alternative splicing of MAG mRNA, which encodes for a protein important for myelin sheath formation and maintenance. QKI has also been implicated in schizophrenia and oligodendrocyte differentiation. QKI can function as a tumor suppressor, as it is regulated by p53 to stabilize miRNAs that regulate TGF-β signaling. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: Neuro-2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 38 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: 1: K562, 2: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDaObserved band size: 38 kDa ICC/IF Cell line: Neuro-2a Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-QKI Rabbit mAb Clone Number: JMMR-3191 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Protein quaking, Hqk, HqkI Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Protein quaking, Hqk, HqkI UniProt ID Q96PU8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:3000 Human, Mouse, Rat ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background QKI is a member of the signal transduction and activator of RNA (STAR) protein family of RNA-binding proteins. Mutations in the mouse Qki locus results in impaired myelin formation resulting in tremors. QKI proteins exist as homodimers, and disruption of the dimerization process is lethal in mice. QKI exists in different isoforms that differ in their C-terminus, resulting in unique subcellular localizations. The nuclear isoform of QKI, QKI-5, is involved in regulation of alternative splicing of MAG mRNA, which encodes for a protein important for myelin sheath formation and maintenance. QKI has also been implicated in schizophrenia and oligodendrocyte differentiation. QKI can function as a tumor suppressor, as it is regulated by p53 to stabilize miRNAs that regulate TGF-β signaling. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: Neuro-2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 38 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: 1: K562, 2: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDaObserved band size: 38 kDa ICC/IF Cell line: Neuro-2a Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with